Mass Spectrometry Questions & Answers

What is DIUTHAME, and why should I try it?

I’m so glad you asked! DIUTHAME is a matrix-free solution for measuring mass spectrum and mass imaging data. What does that mean? That means it’s easy to use, noise-free, and reproducible! If you’ve ever worked with matrix, you know it takes a lot of practice, it introduces noise in the lower mass range, and it’s not able to keep position information.

DIUTHAME stands for Desorption Ionization Using Through Hole Alumina Membrane. (Fun fact: the acronym reminded the Japanese professor, who developed the product, of the work Je t’aime, which means love in French). The combined porous aluminum membrane and laser ionize the sample, and position information can be preserved for imaging. This allows for accurate and reproducible imaging capability. Additionally, the resolution is far superior.

Want to learn more about DIUTHAME? Then watch the videos below.

Video 1: DIUTHAME product introduction

Video 2: How to use DIUTHAME

To see the DIUTHAME products we offer, go to our main website.

What standards have you tested with it?

We provide data on PEO hydrogenated castor oil, peptide mix, polyethylene glycol PEG2000, polystyrene, POPC, Bruker Peptide Calibration Standard II, homogentisic acid, progesterone, cortisol, testosterone, amino acid mix, insulin, black rice, and mouse brain.

How do I prepare my sample? Do I need a special plate, or can I use my standard plate?

Great question! DIUTHAME is very easy to use. For mass spectrum data with liquid samples, you simply drop the sample on the effective area, wait for it to dry, and then adhere the DIUTHAME substrate to your standard plate with the adhesion areas. For imaging data, we recommend you slice the sample 50 µm thick, mount it on an ITO glass slide, and place the DIUTHAME on the sample. Once the sample is absorbed, you can adhere the DIUTHAME to the plate.

Here’s a how-to-use video you might find helpful.

What different form factors can I purchase? Can I reuse a DIUTHAME plate, or is it for one-time use?

We have plates with a 3 mm spot, nine 3 mm spots, or an 18 mm spot for imaging. We just recently came out with a 50 mm x 19 mm plate, which includes a spot for a calibrant. Additionally, we have a new plate for DESI (desorption electrospray ionization). DIUTHAME is for one-time use.

Who is currently using DIUTHAME? Who are you collaborating with?

DIUTHAME was developed by Professor Naito at our partner research university GPI (The Graduate School for the Creation of New Photonics Industries) in Japan. Professor Naito has a long history of work using mass spectrometers, and he developed DIUTHAME as a solution for the drawbacks of matrix. We are very proud of the work that comes out of GPI and honored to have them as our collaborators.

DIUTHAME was originally introduced in Japan in 2015 and introduced to North America at ASMS 2018. Some of our collaborators include Teikyo University, Nagoya University, Institute of Transformative Bio-Molecules (WPI-OTbM), National Institute of Advanced Industrial Science and Technology (AIST), International Mass Imaging Center and Department of Cellular and Molecular Anatomy, and Tohoku University.

We would love to have you try DIUTHAME! Contact us if you would like to try samples.

For more info about DIUTHAME, go to our main website.

If you’ve got a technical question you’d like to see answered on this page, email us.

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Meet the engineers

Columbine Robinson is an Applications Engineer who grew up in lovely California. She’s always loved math and science, and received her Master’s Degree in Solid State Physics from UCSD. Prior to working at Hamamatsu Corporation, she was involved in semiconductor defect inspection where she gained vast experience in optics and image processing. Her hobbies include backpacking/hiking, playing chess, and making pottery.

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